DNA Melting Analysis for Detection of Single Nucleotide Polymorphisms
نویسندگان
چکیده
منابع مشابه
DNA melting analysis for detection of single nucleotide polymorphisms.
BACKGROUND Several methods for detection of single nucleotide polymorphisms (SNPs; e.g., denaturing gradient gel electrophoresis and denaturing HPLC) are indirectly based on the principle of differential melting of heteroduplex DNA. We present a method for detecting SNPs that is directly based on this principle. METHODS We used a double-stranded DNA-specific fluorescent dye, SYBR Green I (SYB...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2001
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/47.4.635